WebSuspended cells were incubated for 30 min in CTL1 antibody, followed by 30 min in anti-IgG FITC-conjugated antibody. Labeled cells were measured for fluorescence using a FACScalibur flow cytometer. WebAug 28, 2024 · CTL1 antibody similarly reduced PC and PE levels (40-50%), further indicating that CTL1 could be involved in the transport of Etn, in addition to its well-characterized function in Cho 14transport (15, 18). [3. H]-Cho and [ C]-Etn transport were similarly inhibited with the CTL1 inhibitor HC-3 (Fig. 1C) and they compete for the same …
Choline transport links macrophage phospholipid metabolism and ...
WebFeb 10, 2012 · CTL1 Colocalizes with Unknown Golgi-Derived Endomembranes That Move in an Actin-Dependent Manner To investigate the subcellular localization of CTL1, we fused a GFP tag in frame to the C terminus of a genomic CTL1 fragment under the control of … WebFurthermore, CTL1 antibody inhibited 14 C-Etn transport in a concentration-dependent manner with an IC 50 of 50 ng (Fig. 1E). CTL2 antibody also inhibited both 14 C-Etn … fisheye opticians islington
Flow cytometry analyses of PMA treatment on CTL1 surface …
WebView Mouse Monoclonal anti-CTL1/SLC44A1 Antibody (1C4) (H00023446-M02). Validated Applications: WB, ELISA, S-ELISA. Validated Species: Human. WebPrimary antibodies Secondary antibodies ELISA and Matched Antibody Pair Kits Cell and tissue imaging tools Cellular and biochemical assays Proteins and Peptides By product … WebJul 20, 2024 · Chronic pharmacological or CTL1 antibody–mediated inhibition of choline uptake resulted in altered cytokine secretion in response to LPS, which was associated with increased levels of diacylglycerol and activation of protein kinase C. These experiments establish a previously unappreciated link between choline phospholipid metabolism and ... can a pet turtle be returned to the wild