WebJan 1, 2024 · Western blot analysis. Approximately 100 mg tissues of pancreas or small intestine were homogenized in RIPA lysis buffer with 1 mmol/L phenylmethylsulfonyl fluoride (PMSF, Biosharp biochemistry Co., Ltd. China) on ice for 30 min, and subsequently centrifuged at 10,000g. 2.9. Statistical analysis WebFeb 9, 2024 · Animal models with mutation of ASD-risked genes, such as methyl-CpG-binding protein 2 (MeCP2), Shank3, Fragile X mental retardation protein 1 (FMRP1), contacting associated protein-like 2 (CNTNAP2), and tuberous sclerosis complex 1/2 (TSC1/2), exhibit a disrupted E/I balance [ 6, 7, 8, 9, 10, 11 ].
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WebSamples, including tissues and cells, were lysed in RIPA lysis buffer (Biosharp) mixed with protease and phosphatase inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) and phenylmethylsulfonyl fluoride (PMSF) (Biosharp, Hefei, China) for 15 min on ice. Then, the proteins were subjected to western blotting according to standard ... WebFeb 21, 2024 · HSFs were lysed with radioimmunoprecipitation assay (RIPA, Beyotime, China) buffer and protease inhibitor (PMSF, Biosharp, China). Then, the proteins were determined by bicinchoninic acid (BCA) assays (Thermo, USA). Furthermore, protein samples were loaded onto 10% SDS-PAGE gels and separated by electrophoresis. clases energy fitness
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