Secondary sphere formation
Web1 Jan 2024 · A sphere is a collection of cells that arise from a single cell through clonal growth. Upon seeding a single stem cell of mammary origin in culture, the first mammosphere to be formed is known as the primary mammosphere. From primary mammospheres, a single cell can be seeded again to generate secondary … Web29 Jul 2013 · Metformin treatment as well as co-treatment with sorafenib markedly impaired primary sphere formation and even more severely impaired secondary sphere formation …
Secondary sphere formation
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Web21 Feb 2024 · This tutorial review showcases recent (2015–2024) work describing ligand construction as it relates to the design of secondary coordination spheres (SCSs). … Web10 Sep 2015 · Secondary sphere formation in the presence of the inhibitor was also further reduced by 6-shogaol. Together, these results indicate that the inhibitory action of 6 …
Webwww.ncbi.nlm.nih.gov Web1 May 2011 · For the primary sphere formation, cells were seeded at 2×10 4 cells/ml and the spheres were analyzed at 7 days in vitro (DIV). For secondary sphere formation, primary spheres were dissociated into a single-cell suspension, seeded at the same density, and cultured under the same conditions for 7 days before counting.
Web1 Mar 2024 · It may be noted that the superstructure of the bipyridine-based second-sphere adduct shown in Figure 2 as a space-filling representation (j) and as a ball-and-stick representation (k) provided (Box 1) inspiration for subsequent research into complex formation between dibenzo-crown ethers and the dicationic bipyridinium herbicides … Web8. For re-plating for colony or secondary sphere formation assay, primary spheres can be dissociated into single cells using Accumax according to the manufacturer's protocol. …
Web18 Sep 2024 · For secondary sphere formation, primary spheres were dissociated with Accutase (A1110501, Thermo Fisher Scientific), followed by 25-gauge needles (Z192406, BD Biosciences) mechanically. Next, 2000 cells/well were replated. Sphere-forming efficiency was calculated as the number of spheres (average diameter = 100 μm) formed divided by …
WebDownload scientific diagram Formation of secondary sphere. (A, B) Secondary spheres were generated after the dissociation of primary spheres derived from peripheral or central keratocytes. Scale ... flagship twitterWeb20 Nov 2013 · We next analyzed the effect of both drugs to hinder sphere formation. As shown in Figure 2F, salinomycin diminished dramatically (p<0.001) the sphere formation ability of LLC cells, whereas the number of spheres was similar to controls when cells were exposed to paclitaxel. A similar effect was found in the human lung cancer cell lines. canon laser toner mf632cdw/634cWebWe applied the repeated sphere formation strategy (cardiac explant → primary cardiosphere (CS) formation → sphere-derived cells (SDCs) in adherent culture condition → … canon lasers imageclass mf6160dwWeb5 Feb 2013 · While several cell lines can be used for tumorsphere formation assay ( e.g. primary mammary tumor cells from Her2/neu -transgenic mice, MCF7, BT474 and … canon laser toner f188300WebThe replating efficiency decreased dramatically between the primary sphere colonies and secondary colonies. When the primary colonies were trypsinized and incubated in serum-free floating culture, secondary colonies were generated (Fig. 1E) , at approximately 15 ± 1 ( n = 3) per dish (10,000 cells). canon law and baptismWeb19 Jun 2012 · Here, we investigated a new strategy that enhances potency and enriches progenitor cells. We applied the repeated sphere formation strategy (cardiac explant → … canon law baptism godparentsWebThe secondary neurospheres are then transferred into a culture medium containing growth factors that promote cell differentiation. The presence of varying cell types, including neurons, astrocytes, and oligodendrocytes, confirms the multipotency of … flagship transportation